Confocal laser scanning microscope
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Confocal imaging with up to 10 fps
- Objectives: 10x, 20x / 63x water, 40x / 100x oil imm., 40x / 63x water dipping
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Spectrometric imaging with four freely selectable emission ranges and settings for any fluorochrome
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line and frame sequential scanning
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Two-photon excitation with internal or non-descanned detectors |
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Arabidopsis leaf double-stained with a DNA- (syto) and a mitochondria-sepcific dye (Rh). Chloroplast-specific autofluorescence is false-coloured in blue. From:
Kristiansen KA, Jensen PE, Møller IM Schulz (2009), Physiologia Plantarum 136:369-383
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Laser lines |
Beam splitter |
Emission |
Detectors |
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351, 364 nm UV |
fast BS |
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458,488,496,514 nm |
freely tuneable AOBS |
freely selectable range |
4 PMT |
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543, 633 nm |
freely tuneable AOBS |
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780-920 nm MP |
fast BS |
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2 NDD |
Alexander Schulz, - last update:24 May 2012
Point Scanning Confocal and 2-photon microscope
SP2 UV MP, Leica Microsystems
Location
Frederiksberg Campus, CAB
Thorvaldsensvej 40, stair 10, 1. floor, room R164c
Typical applications
Live imaging of cells and tissues, (co-) localisation of fluorochromes and fluorescent proteins, 3D confocal imaging (xyz, xyt), Emission scans (xyl) FRET, FRAP
Consultation
Alexander Schulz
Training and problem reporting
Booking
online via PPMS
Rates