Det Biovidenskabelige Fakultet - Københavns UniversitetUniversity of CopenhagenCAB
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Center for Advanced Bioimaging > Instruments > - High-end Point CLSM  
Nørre Campus
- High Content Screening
- Inverted Point Confocal
- High-end FLIM/multiphoton CLSM
- Whole Animal Imager
Frederiksberg Campus
- Super resolution microscope
- High-end Point CLSM
- Point CLSM
- Inverted Point CLSM
- Spinning Disk Confocal
- Histo Lab
- Wide Field Microscopes
- SEM
- High resolution TEM
- Infrared microscope
- Laser Ablation ICP-MS
- Laser Microdissection
Time fees

Confocal laser scanning microscope

  • Confocal imaging with up to 25 fps
  • Objectives: 10x, immersion: 20x 63x water, 40x 63x oil; dipping: 20x 40x 63x water
  • Supercontinuum white light laser (WLL) with up to 8 excitation lines between 470nm and 670nm
  • Two-photon excitation with internal or non-descanned detectors
  • line, frame and stack sequential scanning
  • Spectrometric imaging with five freely selectable emission ranges and settings for any fluorochrome 

SP5 X WLL MP

 Movie 3D

Tobacco leaf mesophylls 3D: movie showing chloroplasts recording in xyz with the resonant scanner, visualised by Volocity software (Liesche et al. unpublished)

 

Laser lines

Beam splitter

Emission

Detectors

355,405 nm UV,V

fast BS

 

458,488,496,514 nm

tuneable AOBS

range freely selectable

5 PMT

470-670 nm WLL

 

 

 

690-1040 nm MP

fas tBS

  

2 NDD

 

 

Alexander Schulz, - last update:25 August 2011
Point Scanning Confocal and 2-photon microscope
SP5-X MP UV, Leica Microsystems

 

Location

Frederiksberg Campus, CAB, Thorvaldsensvej 40, stair 10, 1. floor, room R164d

 

Typical applications

Live imaging of cells and tissues, (co-) localisation of fluorochromes and fluorescent proteins, 4D confocal imaging (xyzt), excitation and emission spectra (l2), photoactivaiton, FRET, FRAP

  

Consultation

Alexander Schulz 

 

Training and problem reporting

 

 

Booking

online via PPMS

 

Rates