Robot microscope

Confocal imaging in 24-, 48-, 96-, 384- or 1536-well plates Environmental control: temperature, CO2, humidity Objectives: 20x and 60x water immersion Three CCD cameras and settings to visualize DAPI, CFP, GFP, YFP, RFP Dispenser unit for addition of compounds to wells Laser based auto focus Script-based Image and Data analysis program that can run during the experiment

 HeLa cells transiently expressing EGFP-ALG-2 (green) and localisation of ER cargo marker p58/ERGIC (red), counterstained with Hoechst to show the nuclei (blue).
Jonas Marstrand La Cour, UCPH BIO, Cell & developmental Biology 

 

Laser lines	Primary Dichroics (beam spitters)	CCD1	CCD2
405 nm	405/488/561/640nm	450/50	540/75
488 nm	405/488/640 nm	520/35	565/40
561 nm	405/561/640 nm	540/75	585/40
640 nm		565/40	600/40