Inverted Confocal
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Inverted confocal microscope
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Objectives: dry: 10x 20x, immersion: 40x 100x oil; 63x water
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Spectrometric imaging with four freely selectable emission settings
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line and frame sequential scanning |
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i) Pancreatic acinar cells loaded with acridine orange. (ii) Intracellular Calcium signals monitored with Fura-2. (iii) Human pancreatic duct cells loaded with calcein (Ivana Novak, unpubl.)
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Laser lines |
Beam splitters |
Emission |
Detectors |
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351, 364 nm UV |
DD488/568, TD 488/568/647 |
freely |
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458,488,496,
514,568,647nm |
TR 30/70, RSP 500
substratum
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selectable emission ranges |
4 PMT |
Alexander Schulz, - last update:27 May 2012
Inverted Point-scanning Confocal
SP/NT UV, Leica Microsystems
Location
Nørre Campus, August Krogh building,
Universitetsparken 13, 6. Floor, room 645
Typical applications
Live imaging of cells and simple tissues, co-localization of proteins and fluorochromes, intracellular ion concentrations, emission scans, xyzt protocols
Consultation
Training and problem reporting