Confocal laser scanning microscope

  • Inverted confocal with  temperature and CO2 control
  • Objectives: 10x, immersion: 20x 63x water, 40x 100x oil
  • 405 nm violet excitation
  • line, frame and stack sequential scanning
  • Spectrometric imaging with five freely selectable emission ranges and settings for any fluorochrome 

SP5 II

 

Arabidopsis root showing colocalisation of a membrane protein and a ER marker (Bangjun Wang, Markus Geisler, Alexander Schulz, unpubl).

 

Laser lines

Beam splitter

Emission

Detectors

405 nm UV,V

freely

 

 

458,476,488,496,514 nm

freely tuneable AOBS

freely selectable range

5 PMTs

 543, 633 nm

freely tuneable AOBS

 


Alexander Schulz, - last update:25 August 2011
Inverted Point Scanning Confocal
SP5 II, Leica Microsystems

 

Location

Frederiksberg Campus, CAB, Thorvaldsensvej 40, stair 2, 1. floor, room D102

 

Typical applications

Live imaging of animal cells and tissues, (co-) localisation of fluorochromes and fluorescent proteins, FRET, FRAP

  

Consultation

Alexander Schulz 

 

Training and problem reporting

 

 

Booking

online via PPMS

 

Rates